Catalog Number - 59050-25P

MicroBee Amino™ is a traditional aminopropyl HPLC stationary phase developed for laboratories requiring a proven alternative to legacy amino column methods. As part of the Cogent™ Similars product line, it has been widely used for many years in carbohydrate analysis, normal phase chromatography, and other applications where amino selectivity is desired. The stationary phase consists of a bonded amino functional group attached to high-purity Type-A silica, providing retention characteristics comparable to traditional amino columns used in established methods.

59050-25P is a 250 mm x 4.6 mm ID analytical column packed with 10 µm, 125Å MicroBee Amino particles. The 250 mm length provides increased resolving power and greater theoretical plates compared to shorter columns, making it well suited for complex separations, method development, and applications requiring maximum chromatographic resolution. The industry-standard 4.6 mm ID format typically operates optimally at approximately 1.0–2.0 mL/min on conventional HPLC systems.

This column is an excellent alternative for methods specifying USP L8 columns, including methods developed on Microbondapak™ Amino and similar stationary phases. Supplied in normal phase solvents, it is optimized for normal phase chromatography and related separations. The aminopropyl functionality provides selectivity useful for carbohydrate analysis, sugar profiling, geometric isomer separations, and other applications where traditional amino phases are preferred.

Key Benefits

• Traditional aminopropyl stationary phase

• Suitable alternative for USP L8 methods

• Greater resolution from 250 mm column length

• Comparable selectivity to legacy amino columns

• Optimized for normal phase chromatography

• 125Å pore size for broad analyte compatibility

• Cost-effective Cogent Similars column

• Suitable for carbohydrate analysis

Typical Applications

• USP L8 methods

• Normal phase HPLC

• Carbohydrate analysis

• Sugar separations

• Food and beverage testing

• Pharmaceutical analysis

• Method development

• Geometric isomer separations

Specifications

• Product Type: HPLC Column

• Stationary Phase: MicroBee Amino

• USP Classification: L8

• Particle Size: 10 µm

• Particle Type: Type-A Silica

• Bonded Phase: Aminopropyl

• Endcapped: No

• Pore Size: 125Å

• Column Dimensions: 4.6 mm ID x 250 mm

• Length: 250 mm

• Recommended Flow Rate: 1.0–2.0 mL/min

• Shipping Solvent: Normal Phase

• Brand: Cogent

Important Notes

• Packed and shipped in normal phase solvents

• Designed primarily for normal phase chromatography

• USP L8 column classification

• Compatible with conventional HPLC systems

• Industry-standard 4.6 mm ID analytical format

• Longer column length provides greater resolving power

• Comparable to traditional Microbondapak™ Amino columns

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